Cyp19a1b-gfp
WebApr 11, 2024 · Here, we have generated a transgenic zebrafish line that expresses green fluorescent protein (GFP) under the promoter of a key senescence marker, p21. We show an increase in p21:GFP+ cells in ... Webcyp19a1a. Predicted to enable several functions, including heme binding activity; iron ion binding activity; and monooxygenase activity. Acts upstream of with a positive effect on …
Cyp19a1b-gfp
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WebNov 20, 2024 · The transgene can be integrated into the host genome by several methods, which are the most common: Transposons, mediated by nucleases such as Zinc Fingers nucleases (ZFNs), Transcription activator-like effector nucleases (TALENs), and RNA-guided nucleases such as endonuclease CRISPR/Cas9 (clustered regularly interspersed … Webcyp19a1b-GFPzebrafish line, we de- termined thatcypa1bup-regulation by E2 is detectable as early as 25 hpf. Altogether these data indicate that thecyp19a1bgene is a target for es- trogens and xenoestrogens at very early stages of development. RESULTS Early Expression of esr1, esr2b, and esr2a mRNAs
WebApr 15, 2016 · Furthermore, E2-EQ values quantified by the in vivo cyp19a1b-GFP assay at these active sites closely relate to the in vitro estrogenic activity quantified by the zfERβ2 assay. Among these sites, the OLL river site was strongly active in zebrafish in vivo and in vitro assays but not in the human assay ( Table 2 , Figure S6), which reinforces a ... WebMay 7, 2012 · The tg(cyp19a1b-GFP) transgenic zebrafish expresses GFP (green fluorescent protein) under the control of the cyp19a1b gene, encoding brain aromatase.
WebAug 15, 2013 · The tg(cyp19a1b-GFP) zebrafish strain is stably transfected with a green fluorescent protein (GFP) gene that is regulated by the zebrafish cyp19a1b (brain … WebFeb 1, 2009 · A cyp19a1b‐gfp (aromatase B) transgenic zebrafish line that expresses GFP in radial glial cells. Aromatase is an enzyme that catalyzes the synthesis of estrogen in …
WebMar 11, 2024 · Newly fertilized cyp19a1b-GFP eleutheroembryos were transferred to glass crystallizers and exposed from 0- to 4-dpf in an incubator (28 ± 1°C) under semi-static conditions with a total renewal of the medium each day. Each condition consisted in a triplicate of crystallizers containing 7 embryos and 15 ml of water.
WebMay 7, 2012 · The tg(cyp19a1b-GFP) transgenic zebrafish expresses GFP (green fluorescent protein) under the control of the cyp19a1b gene, encoding brain aromatase. … hilcorp ticker symbolhilcorp terrebonne bayWebAug 1, 2024 · Selective zfER activation by bisphenols in ZELH-zfER cell lines. • Induction of brain aromatase in transgenic cyp19a1b-GFP zebrafish embryos. • Different estrogenic potency in vitro and in vivo.. BPS could act through a distinct mode of action in vivo on brain aromatase.. Relevance of an integrated strategy to assess EDCs and their substitutes. smallstep downloadWebTg(cyp19a1b:cyp19a1b-GFP) ID ZDB-TGCONSTRCT-110126-4 Name Tg(cyp19a1b:cyp19a1b-GFP) Previous Names None Type engineered_plasmid … smallstep subordinate caWebSep 15, 2016 · The cyp19a1b -GFP transgenic zebrafish ( Tong et al., 2009) were held at the Institut National de l'Environnement Industriel et des Risques (INERIS, Verneuil-en-Halatte, France). They were maintained in 3.5 L aquaria in a recirculation system (Zebtec, Tecniplast, Buguggiate, Italy) on a 14 h light: 10 h dark cycle at a temperature of 25.1 ± … hilcorp sustainability reportWebAlthough high GEN concentrations are necessary to induce cyp19a1b expression in the cyp19a1b-GFP transgenic zebrafish assay, response to GEN seems to be more sensitive compared to another in vivo short-term (48 h) zebrafish embryo assay using morphological defects as endpoints (edema, head and tail malformation, reduced spontaneous … hilcorp webmailWebWith the development of new technologies, new ways of testing chemicals have emerged and will increasingly develop. These new methods generally include Intellectual Property Rights (IPR). IPR aim to stimulate innovation by enabling inventors to appropriate the returns on their investments. smallstep step-ca