Dnazymes: selected for applications
WebApr 10, 2024 · a Schematic of experimental procedure for multiplexed DNA detection. E. coli, S. typhimurium, and S. aureus DNA sensors were labeled with GFP (EC-G), RFP (ST-R) and BFP (SA-B), respectively. The ... WebSep 19, 2024 · DNA-based enzymes, or DNAzymes, are not known to exist in Nature but can be isolated from random-sequence DNA pools using test tube selection techniques. Since the report of the first DNAzyme in 1994, many catalytic DNA molecules for catalyzing wide-ranging chemical transformations have been isolated and studied.
Dnazymes: selected for applications
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WebSep 4, 2024 · DNAzymes: Selected for Applications. Article. Jan 2024; ... Such a method can be generally applied to the detection of other metal ions using other DNAzymes selected through in vitro selection. WebMar 26, 2013 · DNAzymes have shown great promise as a general platform for detecting metal ions, as many metal-specific DNAzymes can be obtained using in vitro selection. While DNAzyme-based metal sensors have found many applications in the extracellular environment, no intracellular application of DNAzyme sensors has yet been reported.
WebAug 29, 2024 · However, most DNAzymes screened are still suffered from the issues of thermodynamic instability and biological instability [32, 33], limiting their application in complex samples and harsh conditions. Therefore, developing a more stable DNAzyme is still an urgent need for its further application in detection. WebOct 28, 2024 · The DNAzyme sequences, however, would be converted to the catalytically active G-quadruplex structure by the presence of target DNA, whose peroxidase mimicking activity then promote the precipitation reaction of 4-chloronaphthol (4-CN) on the electrode.
WebCombating small molecule environmental contaminants: detection and sequestration using functional nucleic acids. Aimee A. Sanford a, Brea A. Manuel a, Misael A. Romero-Reyes * ac and Jennifer M. Heemstra * ab a Department of Chemistry, Emory University, Atlanta, Georgia 30322, USA. WebOct 2, 2024 · The applications of these DNAzymes as biosensors for Zn 2+, as therapeutic agents to cleave intracellular RNA, and as chemical biology tools to manipulate DNA are …
WebMay 21, 2024 · Given that specific RNA-cleaving DNAzymes can be created for diverse bacterial pathogens, direct interfacing of graphene materials with such DNAzymes represents a general and attractive approach for real-time, sensitive, and highly selective detection of pathogenic bacteria. ... and Y. Li: DNAzymes: selected for applications. …
in vivo antioxidant activityWebDNAzymes: Selected for Applications (44 citations) This overview was generated by a machine learning system which analysed the scientist’s body of work. If you have any feedback, you can contact us here. Best Publications Structure-Switching Signaling Aptamers Razvan Nutiu;Yingfu Li. Journal of the American Chemical Society (2003) 919 … in vivo and in situWebMar 25, 2024 · RNA-cleaving DNA enzymes (DNAzymes) have the potential to function as therapeutic agents by silencing the expression of disease-associated proteins. in vivo and in vitro pharmacologyWebMar 13, 2024 · Nine rounds of in vitro selection using an unmodified DNA library were successfully performed to find specific DNAzymes of Vc. Their activity was evaluated based on a fluorescence assay and gel electrophoresis. Finally, a DNAzyme (named DVc1) with good activity and specificity with a detection limit of 7.2 × 10 3 CFU/mL of Vc was … in vivo and philips healthcareWebJan 24, 2024 · Compared with biomedical applications, the work on using DNAzymes as biosensors has advanced more (Gong et al., 2015, Zhang et al., 2011, Zhou et al., 2024b). Lu and coworkers selected the 17E DNAzyme in the presence of Zn 2+ (Li et al., 2000), and it is very similar to the 8-17. in vivo antibody productionWebJun 1, 2024 · Since 2000, the biosensor applications of DNAzymes have emerged and DNAzymes are particularly useful for detecting metal ions, a challenging task for enzymes and antibodies. in vivo antitumor activityWebMar 1, 2024 · DNAzymes are functionally active, synthetic single-stranded (ss) DNA molecules that are capable of catalyzing biological reactions similarly to RNAzymes and proteins. DNA molecules as enzymatic molecules are synthesized using the in vitro selection method known as systemic evolution of ligands by exponential enrichment ( SELEX; see … in vivo and in vitro tests