Fitc dmso

WebDec 1, 2024 · FITC solution in DMSO (5 mg/mL) was added dropwise with gentle stirring to human insulin solution (15 mg/mL) in 0.1 M bicarbonate buffer (pH 9.5) with the molar ratio of FITC:insulin varying from 0.25:1 to 5:1. The reaction mixture was protected from light and kept at room temperature for 0.5–4 h with continuous slow stirring. http://www.delta-f.com/details/1167980

FLUORESCEIN ISOTHIOCYANATE F4274, Isomer I on …

WebDec 11, 2024 · Dissolve FITC in anhydrous DMSO at 1 mg/ml concentration. This should be prepared fresh for each labelling reaction. Add 5 μl of the dye to the dialysed protein (5 … WebFor example, using 5 mg of IgG and a 10∶1 molar ratio of fluorescein isothiocyanate (FITC) with mol wt of 389: (2) 3. Prepare a 10 mg/mL solution of the fluorophore by weighing 3 or more mg of dye and dissolving it in 0.3 mL or more of DMF or DMSO. Sulfonyl ... DMSO, or ethanol. Dilute this solution approx 1∶1000 in the reaction mixture to ... shuteye tracker https://bobtripathi.com

Design and fabrication of fluorescein-labeled starch

Web西安齐岳生物科技有限公司生产销售“磷脂-聚乙二醇-荧光素 dspe-peg2000-fitc”-“产品名称 磷脂-聚乙二醇-荧光素 dspe-peg-fitc 中文名称 荧光素聚乙二醇磷脂 磷脂peg荧光素 英文名称 fitc-peg-dspe dspe-peg-fitc 溶解度 溶于dmso dmf dcm等有机溶剂”，该产品仅用于科研，如果需要请联系我们 WebDMSO: 5 mg/mL DMF: soluble aqueous buffer, pH ≥ 9.0: soluble. storage temp. 2-8°C. ... various proteins, and oligopeptides with fluorescein. FITC-antibody conjugates are useful in many applications, including flow cytometry and immunohistochemistry (excitation maximum = 492 nm; emission maximum = 520 nm). λ max: 492 ± 3 nm (pH 9 ... WebProtocol of conjugation of FITC to peptide amino groups in solution. Reagents: DIPEA/TEA, DMF/DMSO, FITC, peptide. 1. Dissolve peptide (approx. 1 mg/ml; 2 mM or less) and FITC (1.5-3 eq.) in DMF or DMSO. 2. Add TEA or DIPEA (25 eq.) and keep in dark for at least 4 hours. Reaction time can be extended. Control reaction progress using e.g. TLC. 3. shut eye song meaning

DMSO, Anhydrous - Thermo Fisher Scientific

Category:FITC, Isomer I Fluorescein Isothiocyanate, Isomer I 3326-32-7

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Fitc dmso

FLUORESCEIN ISOTHIOCYANATE F4274, Isomer I on …

WebFluorescein isothiocyanate ( FITC) is a derivative of fluorescein used in wide-ranging applications [1] [2] including flow cytometry. First described in 1942, [3] FITC is the … WebAttach a 20-gauge needle to the tip of a 1 mL syringe. Use this syringe to extract the desired volume of DMSO (use 100 µL per mg of NHS-ester modification solid; depending on the modification, additional DMSO may be necessary). Use the “balloon” technique to maintain the DMSO stock bottle under nitrogen pressure and thereby keep moisture out.

Fitc dmso

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WebB. Preparing FITC Dilutions Dissolve the FITC (250 mg/ bottle) in 4 mL of DMSO, by pipetting the DMSO into the container. Leave the container in a dark place at room temperature for 2 hours (or until all the FITC has dissolved.) Pour the entire dilution into a 500 mL VWR bottle. Add enough DMSO to fill the bottle to the 500 mL mark. WebDMSO: 10 mg/mL (2.5mM), Need ultrasonic ( < 1 mg/ml refers to the product slightly soluble or insoluble ) 参考文献. 1. Eriksson I, et al. Analysis of Lysosomal pH by Flow Cytometry …

WebFITC is a fluorescent reagent for the labeling of amines. Excitation maximum ~ 495 nm; emission maximum ~ 525 nm. Optical Data for FITC. ... Soluble to 100 mM in DMSO. Purity: ≥95%. Storage: Store at -20°C. CAS No: … WebFITC is generally supplied as a lyophilized solid and must be resuspended in an organic solvent, such as ethanol, DMSO, or DMF, before labeling proteins or any other amine …

WebAug 5, 2013 · The FCS study reveals a change of the hydrodynamic radius of BSA from 3.7 nm in the native state to 7.0 nm in the presence of 40% DMSO, which suggests complete unfolding of the protein under these conditions. Fluorescence self-quenching of FITC has been exploited to understand the conformational dynamics of BSA. WebApr 11, 2024 · 荧光素聚乙二醇巯基 FITC-PEG-SH-德尔塔(Delta)生物试剂定制生产，产品长期供货。荧光素聚乙二醇巯基 FITC-PEG-SH-德尔塔(Delta)生物试剂仅用于科研。荧光素聚乙二醇巯基 FITC-PEG-SH-德尔塔(Delta)生物试剂不能用于人体治疗、药物开发、和其他商业用途。如果需要采购荧光素聚乙二醇巯基 FITC-PEG-SH-德尔塔 ...

WebDimethyl sulfoxide, or DMSO, is a highly polar organic solvent that is miscible with water. ... (FITC)) • Thiol-reactive fluorescent labeling reagents (e.g. Alexa Fluor™ 488 or fluorescein maleimide) Molecular Probes™ …

WebFITC (fluorescein-5-isothiocyanate) is an amine-reactive fluorescein derivative for labeling proteins or other molecules containing primary amines. λ Ex /λ Em = 492/520 nm. ε = 75,000. Orange solid soluble in DMF or DMSO. Store at -20°C and protect from light, especially in solution. shut eye tv show castWebMar 30, 2024 · 溶剂：溶于dmso、dmf，dcm，溶于水。 ... fitc-peg-nh2是一种线性异生物功能peg试剂，含有荧光素染料和胺基，与羟基甲酸、活化nhs酯、羰基（酮、醛）等反应。荧光素通常称为fitc，是生物学中常用的荧光示踪剂，用于染色细胞、组织、生物标记物或纳米 … the pact online subtitratWebLoudoun Benefits Office. Service Area. Ashburn, Aldie, Dulles, Leesburg, Loudoun, Purcellville, Sterling, South Riding, Loudoun County, Frederick County, Hamilton, and … shut eye tv series castWebAug 1, 2011 · Preparation of fluorescein labeled SA (FI-SA) One gram of the SA dissolved in 10 mL of DMSO was reacted with 25 mg of FITC in the presence of 1.5 mL DBTDL for 5 … shuteyface2WebNov 22, 2011 · In vitro disease modeling using pluripotent stem cells can be a fast track screening tool for toxicological testing of candidate drug molecules. Dimethyl sulfoxide (DMSO) is one of the most commonly used solvents in drug screening. In the present investigation, we exposed 14- to 21-day-old embryoid bodies (EBs) to three different … the pact on rokuWebFITC-dextran is supplied as a yellow/orange powder which dissolves freely in water or salt solutions giving a yellow solution. The product also dissolves in DMSO, formamide and certain other polar organic solvents but is insoluble in lower aliphatic alcohols, acetone, chloroform, dimethylformamide. shut eye tv show onlineWebThey suggest to prepare protein solution not less than 2 mg/ml and add 50 ul of FITC (1 mg/ml in DMSO) per 1 ml of protein solution. Final molar F/P ratio was 0.06. In another … the pact on stan