Pdk intron rnai
Splet12. apr. 2010 · Finally, the Pdk intron fragment of pPDK was ligated into ApaI site of pYL41-ccdB to generate pRNAi-LIC. pRNAi-LIC was used to make all ihpRNA constructs for … Splet21. sep. 2000 · Post-transcriptional gene silencing (PTGS), a sequence-specific RNA degradation mechanism inherent in many life-forms, can be induced in plants by …
Pdk intron rnai
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Splet23. mar. 2024 · The PDK intron contained within the RNAi hairpin ( Wesley et al., 2001) was amplified using the primers Pdk-F (containing XmaI and XbaI site) and Pdk-R (containing SacI and BamHI sites) and then inserted between the promoter and terminator by the XmaI and BamHI restriction sites ( Supplementary Figure S5B ). Splet25. mar. 2024 · RNAi is also known as Post-Transcriptional Gene Silencing (PTGS), in which the target RNA degradation occurs in a sequence-specific manner post-formation of double-stranded RNA, and its processing into small-interfering RNAs (siRNA) by the Dicer-Like (DCL) proteins and the RNA-Induced Silencing Complex (RISC) [ 24, 25, 26, 27, 28, 29 ].
Splet结果表明,RNAi的中间载体和表达载体均结构正确,并且RNAi表达载体p ART27-Lb CER1 (+)-PDK intron-Lb CER1 (-)成功转化根癌农杆菌GV3101,说明该构建方法正确,将为进一步筛选Lb CER1-RNAi植株,获得适宜干制加工的宁夏枸杞新品种奠定基础。 下载App查看全文 下载全文 更多同类文献 论文一键排版服务>> PDF全文下载 CAJ全文下载 ( 如何获取全文 ？ 欢迎： … SpletRNAi provides a powerful tool for functional genomics due to its targeted nature, silencing of genes to the equivalent of null mutants and applicability to any transformable species. …
SpletpHANNIBAL Sequence and Map pHANNIBAL Vector for silencing genes in plants using intron-containing hairpin RNA (hpRNA). Open in SnapGene Try SnapGene for Free … Splet01. jan. 2005 · Map of pHELLSGATE12. pHELLSGATE12 is identical to pHELLSGATE8 with the catalase-1 intron (8) inserted between the PDK intron and the second attR cassette in the antisense orientation with ... Systematic functional analysis of the Caenorhabditis elegans genome using RNAi. Nature, 421 (2003), pp. 231-237. View in Scopus Google …
SpletNC frame-PDK intron-inverted NC frame. How to cite this plasmid ( Back to top) These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications. ... pNC-Cam1304-RNAi was a gift from Pu ...
Splet16. jan. 2014 · PDK intron—a pyruvate dehydrogenase kinase intron; ... dTTP and dCTP mix, 2.5 μl 50 ng ml −1 of each RNAi vector-specific 35S-F/PDK-R or PDK-F/OST-R primers pairs ... gift box with ribbon bulkSpletIntron-mediated RNA interference, intronic microRNAs, and applications. Nearly 97% of the human genome is non-coding DNA. The intron occupies most of it around the gene … gift box wrapping christmasSplet25. mar. 2024 · The PDK intron was cloned from pRNAi-LIC and ligated into the pGEM-T Easy vector. The ihpRNAi construct is made by assembling the sense and antisense … fryeburg post office hoursSpletthroughput cloning of the intron-containing inverted repeat inserts into the vectors, we developed a new plant T-DNA RNAi vector, pRNAi-GG. This vector consists of a ccdB gene - Pdk intron - ccdB gene cassette between the duplicated CaMV 35S promoter and Nos terminator (Fig. 1A). One BsaI recognition site, in the original gift box wrapping harry potterSplet(A) Schematic representation of the RNAi vector. P 35S , CaMV 35S promoter; hyg r , hygromycin resistance gene; Intron, the Pdk intron; PLR, the 400–500 bp fragment of the coding region of... fryeburg post office phone numberSplet16. jan. 2014 · PDK intron—a pyruvate dehydrogenase kinase intron; blue boxes represent att sites attached to the target gene fragment. Full size image Figure 2: Phytochrome … fryeburg police maineSpletData Plane Development Kit (DPDK*) This open source application is a set of libraries and drivers that accelerate packet processing and the ability to create packet forwarders … fryeburg police